| | Category | CH | P05 | Biodegradable Nanoparticles: Drug Delivery System for Cancer |
| | Abstract | The aim of my project is to develop biodegradable nanoparticles, an |
| | anticancer drug delivery system so when the anticancer drug is delivered |
| | to the target the system will be completely metabolized without any toxic |
| | metabolites. This will reduce the burden of toxic waste accumulating inside |
| | the human body. |
| | |
| | The effect of polymer ratio, pH, and agitation speed on the formation |
| | of nanoparticles was studied. Nanoparticles were created by mixing |
| | weight to weight ratios of protamine and dextran sulfate (1:1, 1:2, 1:3) |
| | using zinc sulfate as a cross linking agent. pH buffer solutions were |
| | added to each weight ratio samples to create pH 5, 7 and 8 mediums. Each |
| | sample was loaded with doxorubicin. Samples were agitated for 5 |
| | minutes with the magnetic stirrer at 600rpm, 1000rpm, and 1400rpm values |
| | at room temperature. |
| | |
| | My project confirmed that with the 1:1 weight ratio of Protamine |
| | sulfate and Dextran sulfate at a neutral pH of 7 and agitation speed of |
| | 1400 rpm at room temperature produced nanoparticles with an ideal size |
| | of 205nm that is within the recommended range of 100-250nm. |
| | Nanoparticles were further characterized by dynamic light scattering zeta |
| | plus analyzer and obtained Polydispersity Index (0.7080) and Zeta |
| | Potential (-34.44mV) values at the size of 205nm. A higher value in |
| | polydispersity index and zeta potential confirms the stability of the |
| | nanoparticles in the colloidal system. I analyzed my data with Analyse-it® |
| | Statistical Model and obtained p value of 0.0001 which is statistically |
| | significant. |
| | |
| | This concludes it is possible to create stable biodegradable |
| | nanoparticles. |
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| | Particle Sizing |
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| | Menu. 27 June 2008 |
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| | e=1&print=1>. |
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